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Media formulation

Media formulation
The answer must be on your one word and short answer no more than 5-6 lines. each question must have references.
1- In microbiology, you autoclave growth media to ensure it is sterile. Why
do we not autoclave cell culture media? How do we ensure the
continued sterility of cell culture media?
2-What is PBS and how is it used in animal cell culture?
3- How do we sterilize the PBS?


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